Director: Martina L. Veigl, PhD
Associate Professor, General Medical Sciences (Oncology)
mlv2@case.edu

Link to the Gene Expression and Genotyping Core Facility home page: http://www.gegf.net.

Remember to acknowledge the valuable services provided by Cancer Center Core Facilities in your research papers and publications:
"This research was supported by the Gene Expression and Genotyping Core Facility of the Case Comprehensive Cancer Center (P30 CA43703)."

Services

The Gene Expression and Genotyping Facility (GEGF) facilitates implementation of high throughput genetic technologies for the research conducted by members of the Case community.

Primary Services

Gene Expression (Affymetrix)

• Brokerage of microarrays (testchips and full expression arrays)
• cRNA preparation
• Evaluation of cRNA quality on test arrays
• Processing user-prepared and/or facility-prepared cRNA microarrays
• Data reduction/data analysis
• Low cost access to valuable, advanced, server-based data mining and annotation tools

Gene Expression (RT PCR)

• Brokerage of ABI RT PCR assay kits and reagents
• Sample preparation
• RT PCR set-up for relative quantitation and absolute quantitation
• Execution of RT PCR for user-prepared and/or facility-prepared PCR reactions
• Help with data interpretation/analysis
• Endogenous control determinations

SNP Analysis (Affymetrix)

• Brokerage of 10K and 100K SNP arrays
• Sample preparation
• Processing of sample on Affymetrix SNP array
• Help with analysis

SNP Analysis (ABI)

• Sample prep (384 well format, high throughput enabled by Matrix robotics)
• Endpoint plate reading (high throughput enabled by ABI autoloader)
• Measurement of real time spectral data (initial validations) on 7900
• Basic PCR execution on 9700
• Matrix robotics (programmable for custom set-up)
• Help with analysis

Accurate RNA quality assessment and quantitation

• Accurate quantitation of RNA samples on Nanodrop spectrophotometer
• Quality assessment on Agilent Bioanalyzer

Additional Services

• Individually tailored consultation with potential users
• Assistance with grant preparation
• Assistance with experimental design
• Facilitate monthly meetings of Working User's Group to discuss experimental design, data reduction, etc.
• Serve as resource for linking users to databases, expression array data, etc.
• Provide 3 credit hour course on issues pertaining to expression arrays
• Regular software workshops/training

Fees

Visit http://www.gegf.net to obtain fees for Arrays and Services.

Contact for Information