GENE EXPRESSION and GENOTYPING CORE FACILITY
Director:
Martina L. Veigl, PhD
Associate Professor, General Medical Sciences (Oncology)
mlv2@case.edu
Link to the Gene Expression and Genotyping Core Facility home page here.
Services
The Gene Expression and Genotyping Facility (GEGF) facilitates implementation of high throughput genetic technologies for the research conducted by members of the Case community.
Primary Services
Gene Expression (Affymetrix)- Brokerage of microarrays (testchips and full expression arrays)
- cRNA preparation
- Evaluation of cRNA quality on test arrays
- Processing user-prepared and/or facility-prepared cRNA microarrays
- Data reduction/data analysis
- Low cost access to valuable, advanced, server-based data mining and annotation tools
- Brokerage of ABI RT PCR assay kits and reagents
- Sample preparation
- RT PCR set-up for relative quantitation and absolute quantitation
- Execution of RT PCR for user-prepared and/or facility-prepared PCR reactions
- Help with data interpretation/analysis
- Endogenous control determinations
- Brokerage of 10K and 100K SNP arrays
- Sample preparation
- Processing of sample on Affymetrix SNP array
- Help with analysis
- Sample prep (384 well format, high throughput enabled by Matrix robotics)
- Endpoint plate reading (high throughput enabled by ABI autoloader)
- Measurement of real time spectral data (initial validations) on 7900
- Basic PCR execution on 9700
- Matrix robotics (programmable for custom set-up)
- Help with analysis
- Accurate quantitation of RNA samples on Nanodrop spectrophotometer
- Quality assessment on Agilent Bioanalyzer
Additional Services
- Individually tailored consultation with potential users
- Assistance with grant preparation
- Assistance with experimental design
- Facilitate monthly meetings of Working User's Group to discuss experimental design, data reduction, etc.
- Serve as resource for linking users to databases, expression array data, etc.
- Provide 3 credit hour course on issues pertaining to expression arrays
- Regular software workshops/training
Fees
Click here to obtain fees for Arrays and Services. There is a 30% discount for Cancer Center members.
Contact for Information
| Location: | Wolstein Research Building, 3-401 |
| Director: | Martina L. Veigl, PhD |
| Phone: | 216/368-5689 |
| E-mail: | mlv2@case.edu |
| Manager: | Debora Poruban |
| Phone: | 216/368-8748 |
| E-mail: | debora.poruban@case.edu |