CYTOMETRY CORE FACILITY
Director: James W. Jacobberger, PhD
Professor, General Medical Sciences (Oncology)
jwj@case.edu
Services
The Cytometry core provides flow and image cytometry and cell sorting instrumentation, expertise, training, consultation, and services to Cancer Center investigators. Services provided:
- Consultation
- Training
- Teaching
- Flow Cytometry – low, intermediate and high levels
- Image Cytometry
- Cell Sorting
- Cell Counting, live/dead measurements
- Data Analysis
- Staining
Instrumentation
Cell Sorters:
- BD Biosiences FACSAria: 3 laser, 9 parameter, high speed, 4 way, auto-cloning, ~biosafety II
- Beckman Coulter Elite ESP: 2/3 laser, 8 parameter, normal speed, 2 way, auto-cloning
Cell Analyzers:
- BD Biosiences LSR: 3 laser, 8 parameters (6 color)
- BD Biosiences LSR 2: 4 laser, 42 parameters (12 color)
- Beckman Coulter XL: 1 laser, 8 parameter (4 color)
Compucyte iCyte:
- 3 laser, solid support scanning cytometer (6 parameter, 5 color)
Resources and Fees
| Instrument | Hourly Rate | ||
| Room | CC Member | Non-CC Member | |
| Cell Sorting | |||
| BD FACSAria (3 Laser Digital Sorter) | 3-517 | 100.00 | 130.00 |
| iCyt Reflection (5 Laser Digital Sorter) | 3-517 | 100.00 | 130.00 |
| Laser Scanning Cytometer | |||
| Compucyte iCyte | 3-517 | 100.00 / Run | 130.00 / Run |
| Analyzers | |||
| BD LSR I (3 Laser Analyzer) | 3-515 | 75.00 | 98.00 |
| BD LSR II (4 Laser Digital Analyzer) | 3-515 | 90.00 | 117.00 |
| BC XL-MCL (1 Laser Analyzer) | 3-515 | 60.00 | 78.00 |
| Counters | |||
| Guava EasyCyte | 3-517 | 1.00 / Test * | |
| * Test reagants available at additional cost | |||
Contact for Information
| Location: | Wolstein Research Building-3rd Floor, Cell Sorters-3515, Cell Analyzers-3517 also at the Lerner Research Institute |
|---|---|
| Director: | James W. Jacobberger, PhD |
| Phone: | 216/368-4645 |
| E-mail: | jwj@case.edu |
| Manager: | R. Michael Sramkoski |
| Phone: | 216/368-1021 |
| E-mail: | rms19@case.edu |