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Tissue Procurement, Histology, & Immunohistochemistry Core Facility
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Director: Gregory T. MacLennan, MD
Professor, Pathology
gregory.maclennan@UHhospitals.org
Co-Director of IHC: Dawn M. Dawson, MD
Assistant Professor, Pathology
dawn.dawson@UHhospitals.org
Remember to acknowledge the valuable services provided by Cancer Center Core Facilities in your research papers and publications:
"This research was supported by the Tissue Procurement, Histology, & Immunochemistry Core Facility of the Case Comprehensive Cancer Center (P30 CA43703)."
Services
The Core facility supports Cancer Center member researchers by acquiring, preserving, and distributing high quality human tissues and providing priority histology, immunohistochemistry, and tissue microarray services for both human and animal tissues. Services are open to all Cancer Center members on an equal basis. Free parking is provided to members picking up samples who work off the CWRU/University Hospitals campus.
Services available:
Tissue Procurement
- Consulting services on tissue availability and researcher application process
- Information source regarding Institutional Review Board (IRB) requirements for research uses of discarded human tissues and patient privacy regulations (HIPAA)
- Normal, benign, diseased, and malignant tissues from surgeries and autopsies
- Matched normal adjacent tissue and tissues from different organ sites of the same donor with special emphasis on accessing metastatic samples
- Tissues are prepared according to user-defined protocols and can be fresh, snap-frozen, or fixed
- Protocol development for tissue collection and processing
- Quality control reviews on human tissue samples are conducted to verify diagnosis before release to investigators (unless needed immediately)
- Demographic (age, race, gender) and histopathologic data supplied at no charge
- Long-term, monitored storage of samples (over 3 months)
Histology
- Hematoxylin and Eosin (H&E) staining, special morphological stains, and enzyme histochemistry available upon request
- Paraffin, OCT or methacrylate embedding
- Standard and serial sectioning
Immunohistochemistry
- Routine immunohistochemistry and protocol development of commercially available and novel antibodies
- Custom design and production of Tissue Microarray (TMA) blocks
- Basic light, fluorescence, and phase contrast microscopy with digital imaging for CCC Histology, IHC, & TMA prepared slides
- In situ hybridization service in development – Please Inquire!
Please note: Researchers must complete a formal application and have IRB approval to access human tissue from the Core.
Resources
- Fully equipped laboratories for tissue collection, processing, histology, and immunohistochemistry work
- Four liquid nitrogen freezers with 18,000 total sample capacity, equipped with electronic monitoring, automated fill and alarm systems
- Workstations for data collection and management; access to hospital databases
- Daily access to Tissue Tek VIP automatic tissue processor
- BioCare Medical Nemesis 7200 IHC Automated Slides Stainer (continuous access, 72-slide capacity)
- BioCare Medical IQ3000 Kinetic Slide Stainer for temperature controlled incubations and in situ hybridization protocols (36-slide capacity)
- Pathology Devices Semi-Automated Tissue Microarray Maker (TMArrayer) for both paraffin embedded and frozen tissues
- Instrumedics Paraffin Tape Transfer System for sectioning and optimal tissue recovery from TMA blocks
- Olympus BX41 Microscope with motorized scanning stage, light, phase contrast, fluorescence, and digital imaging capabilities
- Two Leica 1850 Cryostats and one 1800 Cryostat all with Peltier cooling for preparation of frozen section slides
- Leica RM2255 Full-Automated Microtome and several other microtomes for cutting of blocks and preparation of paraffin section slides
- Hacker-Bright Model 5030 Microtome for cutting of blocks and preparation of methacrylate section slides
Fees
| Locations: | Wearn Building, Rm. 333 (Tissue Procurement and IHC) |
|---|---|
| and Institute of Pathology, Rm. B-12 (Histology) | |
| Director: | Gregory T. MacLennan, MD |
| Phone: | 216.844.7452 |
| E-mail: | gtm2@case.edu |
| Co-Director of IHC: | Dawn M. Dawson, MD |
| Phone: | 216.368.5912 |
| E-mail: | dawn.dawson@UHhospitals.org |
| Director of Operations: | Robert E. Wyza, MS |
| Phone: | 216.844.5389 |
| Fax: | 216.844.4911 |
| E-mail: | rew7@case.edu |
| Histology Manager: | Nancy L. Edgehouse, BS |
| Phone: | 216.368.8700 |
| E-mail: | nle@case.edu |
| IHC Services: | Adam Kresak, BS |
| Phone: | 216.844.0076 |
| E-mail: | amk41@case.edu |